Msx2 promotes cardiovascular calcification by activating paracrine Wnt signals
J. Clin. Invest. Jian-Su Shao, et al. 115:1210 doi:10.1172/JCI24140 [Go to this article.]

Figure 3
CM from Msx2-expressing 10T1/2 cells enhances ALP activity and suppresses adipogenesis. C3H10T1/2 cells were transduced with retroviruses expressing either Msx2 or LacZ, and CM was harvested. Subsequently, naive 10T1/2 cells were induced to undergo either osteogenic or adipogenic differentiation in the presence of CM from either control LacZ cells or Msx2-expressing cells. (A) CM from Msx2-expressing cells supports significantly higher levels of ALP activity than CM from control cells. Percentages indicate the percent of the total cultured cell population that stains with oil red O. (B) CM from Msx2-expressing cells suppresses adipogenesis as measured by oil red O staining. (C) 10T1/2 cells were transiently transfected with expression vectors encoding either EGFP or EGPF-Msx2 fusion protein. Four days later, cells were fixed, and dual immunofluoresence was carried out as previously described, using Vector Red to image ALP. Arrows indicate cells expressing either EGFP or EGFP-Msx2 fusion proteins (green, EGFP-Msx2 in nuclear compartment). Intense ALP staining appears adjacent to cells expressing EGFP-Msx2. *P < 0.01