Negative regulation by thyroid hormone receptor requires an intact
coactivator-binding surface
J. Clin. Invest. Tania M. Ortiga-Carvalho, et al. 115:2517 doi:10.1172/JCI24109 [
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Figure 5Effect of TH deficiency and excess on regulation of the H-P-T axis. (
A) Serum TSH levels were sequentially determined in
TR-β
WT/WT,
TR-β
E457A/WT, and
TR-β
E457A/E457A mice after they had been on the LoI/PTU diet and given MMI water for 5 weeks and had received the additional treatment with different doses of L-T
3 for 5 days each. *
P < 0.01 vs. WT. (
B) Total serum T
3 levels at the end of the L-T
3 treatment. (
C and
D)
Tshb and
Tsha mRNA levels in the anterior pituitary of
TR-β
WT/WT,
TR-β
E457A/WT, and
TR-β
E457A/E457A mice using a quantitative RT-PCR analysis. Data were normalized for each mRNA level relative to values for the WT animals. Five to 10 animals were evaluated in each group, and data are shown as mean ± SEM.
