Negative regulation by thyroid hormone receptor requires an intact
coactivator-binding surface
J. Clin. Invest. Tania M. Ortiga-Carvalho, et al. 115:2517 doi:10.1172/JCI24109 [
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Figure 2Characterization of TR-β (E457A) mice. (
A) Introduction of E457A mutation into the mouse TR-β gene by homologous recombination. KI, knock-in. (
B) Homologous integration of the targeting vector was confirmed by Southern blot analysis of genomic DNA digested with BamHI and probed with a probe located outside the targeting vector. (
C) RT-PCR of TR-β from pituitary RNA using primers described in Methods. A 950-bp fragment from the
TR-β
WT/WT,
TR-β
E457A/WT, and
TR-β
E457A/E457A mice was observed. Two different animal tissues from each group were analyzed. (
D) DNA sequence of the 950-bp fragment. The mutated amino acid is underlined. (
E)
Thrb and
Thra mRNA levels in the anterior pituitary of
TR-β
WT/WT,
TR-β
E457A/WT, and
TR-β
E457A/E457A mice according to quantitative RT-PCR analysis. Data were normalized for each mRNA level relative to the values for the WT animals (
n = 5–8).
