Loss of IRF-4–binding protein leads to the spontaneous development of systemic autoimmunity
J. Clin. Invest. Jessica C. Fanzo, et al. 116:703 doi:10.1172/JCI24096 [
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Figure 5IBP regulates T cell effector function. (
A) IL-2 production by WT and IBP mutant T cells. Purified CD4
+ T cells were stimulated with immobilized anti-CD3ε antibody (1 μg/ml) alone or together with soluble anti-CD28 antibody (1 μg/ml) (left panel) or with PMA (50 ng/ml) and ionomycin (1 μM) (right panel) for 24 hours. IL-2 levels in culture supernatants were determined by ELISA. The experiment is representative of 5 independent experiments. (
B) IFN-γ and IL-4 production by WT and IBP mutant T cells. Cells were stimulated as in
A for 48 hours. Production of IFN-γ (left panel) and IL-4 (right panel) was measured by ELISA. The experiment is representative of 5 independent experiments. (
C) In vitro differentiation of
IBP+/+ and
IBPtrap/trap naive Th cells. Naive CD4
+ T cells were isolated from WT and
IBPtrap/trap mice and differentiated in vitro under unskewed, Th1, or Th2 conditions. After 7 days, unskewed, Th1, and Th2 cells from
IBP+/+ and
IBPtrap/trap mice were stimulated with anti-CD3 antibody for 24 hours and supernatants analyzed for cytokine production. IFN-γ (left panel) and IL-4 (right panel) production was measured by ELISA. The experiment is representative of 5 independent experiments.
