Loss of IRF-4–binding protein leads to the spontaneous development of systemic autoimmunity
J. Clin. Invest. Jessica C. Fanzo, et al. 116:703 doi:10.1172/JCI24096 [Go to this article.]

Figure 3
Accumulation of effector/memory-like T cells and terminally differentiated B cells in aging IBP^trap/trap mice. (A) Increased effector/memory T cells in aging IBP^trap/trap female mice. Cell suspensions from spleen (top panels), and lymph nodes (bottom panels) from older IBP^+/+ and IBP^trap/trap mice were stained with antibodies against CD4, CD44, and CD62L to evaluate the presence of naive (CD44^loCD62L^hi) and effector/memory (CD44^hiCD62L^lo) T cells. Dot plots represent gated CD4⁺ populations. Percentages of positive cells within each gate are shown. Results are representative of 5 different mice. (B) Alterations of B cell populations in aging IBP^trap/trap female mice. Single-cell suspensions from spleen (top panels) and lymph nodes (bottom panels) from aging IBP^+/+ and IBP^trap/trap female mice were stained with antibodies against B220, IgM, and IgD. Cell staining was analyzed by FACS. Dot plots represent gated B220⁺ populations. Percentages of positive cells within each gate are shown. (C) Increased plasma cells in spleens of aging IBP^trap/trap female mice. Single-cell suspensions from spleen (top panels) and bone marrow (bottom panels) from IBP^+/+ and older IBP^trap/trap female mice were stained with antibodies against B220 and syndecan-1. Cell staining was analyzed by FACS. Percentages of syndecan-1–positive cells within each gate are shown. (D) Syndecan-1 and PNA staining of spleens of aging IBP^trap/trap female mice. Anti–syndecan-1 (blue) and anti-PNA (red) staining were performed on spleens from IBP^+/+ (left panel) and IBP^trap/trap (right panel) mice. Light microscopy magnification, ×10. (E) Accumulation of IgG1⁺ cells in spleens of aging IBP^trap/trap female mice. Anti-IgG1 (blue) staining was performed on spleens from IBP^+/+ (left panel) and IBP^trap/trap (right panel) mice. Light microscopy magnification, ×10.