Loss of IRF-4–binding protein leads to the spontaneous development of systemic autoimmunity
J. Clin. Invest. Jessica C. Fanzo, et al. 116:703 doi:10.1172/JCI24096 [
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Figure 2Aging
IBPtrap/trap female mice develop a lupus-like syndrome. (
A) Spleens (left panel) and lymph nodes (right panel) of 7-month-old
IBP+/+ and
IBPtrap/trap female mice are shown. (
B) Total serum IgG levels in older (5–12 months old)
IBP+/+ (filled circles) or
IBPtrap/trap (open circles) mice were determined by ELISA. Each symbol represents 1 mouse of
IBP+/+ females (
n = 7),
IBPtrap/trap females (
n = 13),
IBP+/+ males (
n = 6), and
IBPtrap/trap males (
n = 6). (
C) ANA titers were determined from
IBP+/+ (filled circles) or
IBPtrap/trap (open circles) female mice (5–12 months old) by measuring the intensity of fluorescent staining of ANAs on a scale ranging from 0 to 4, with 4 being the highest intensity. Data shown represent
IBP+/+ female (
n = 8) and
IBPtrap/trap female mice (
n = 13). (
D) Anti-dsDNA antibody titers in the serum of older (5–12 months old)
IBP+/+ (filled circles) or
IBPtrap/trap (open circles) mice were determined by ELISA. Each symbol represents 1 mouse of
IBP+/+ females (
n = 7),
IBPtrap/trap females (
n = 16),
IBP+/+ males (
n = 4), and
IBPtrap/trap males (
n = 6). (
E) Histological analysis of H&E-stained sections from the kidney of 7-month-old
IBP+/+ and
IBPtrap/trap female mice (upper panel). Light microscopy magnification, ×40. Deposition of Ig complexes in glomeruli of
IBPtrap/trap mice as detected by immunofluorescence with anti-IgG (middle panel) and anti-C3 (bottom panel) staining. Results are representative of 5 mice.
