ATP and purinergic receptor–dependent membrane traffic in bladder umbrella cells
J. Clin. Invest. Edward C.Y. Wang, et al. 115:2412 doi:10.1172/JCI24086 [Go to this article.]

Figure 2
Effects of ATP, apyrase, and PPADS on changes in membrane capacitance. (A and B) Isolated rabbit uroepithelial tissue was exposed to pressure alone (Pressure), pressure and 0.4 U/ml apyrase (Apyrase + pressure), or pressure and 100 μM PPADS (PPADS + pressure). PPADS and apyrase were added to either the serosal (A) or mucosal (B) surfaces of the tissue. The mean changes in capacitance ± SEM (n ≥ 3) are shown. *Statistically significant difference (P < 0.05) relative to the samples treated with pressure alone. (C) ATP (at the indicated concentration) was added to the serosal surface of isolated rabbit uroepithelium without pressure stimulus. The mean change (± SEM) in capacitance at 300 minutes after addition of ATP was recorded (n ≥ 3) and plotted versus the log of the ATP concentration.