Inflammation-induced lymphangiogenesis
in the cornea arises from
CD11b-positive macrophages
J. Clin. Invest. Kazuichi Maruyama, et al. 115:2363 doi:10.1172/JCI23874 [
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Figure 7Behavior of PEC in a tube-formation assay. (
A and
B) Matrigel assay at day 3 containing 10
5 cells/ml. (
A) PECs appear to be aligning end-to-end in the assay (arrowheads). (
B) PECs associating to form a vessel-like structure. (
C) Measurement of aggregation and tube-like structure field in the Matrigel assay at day 2. (
D–
S) Long-term (31 days) assay containing 2 × 10
6 cells/ml. (
D and
E) Micrograph of the tube-like structure in the Matrigel (31 days). (
F–
H) Another area of a Matrigel assay (day 31) containing 2 × 10
6 cells/ml. Shown are sequential images of upper (
G), middle (
H), and lower (
I) levels of matrigel cultures. (
J) Horizontal view of images in
G–
I. (
K,
M, and
O) Matrigel assay at day 31 containing 2 × 10
6 cells/ml. (
L and
N) Cy3-visualized podoplanin staining and nuclear staining with DAPI (blue). (
P) Fluorescence microscopy image of matrigel cultures. Arrow depicts area evaluated by confocal microscopy in
Q–
S. (
Q–
S) Confocal image of Matrigel at day 31. EGFP expression (
Q) and Cy3-visualized LYVE-1 staining and nuclear staining with DAPI (
P and
R). (
S) Overlay of
Q and
R showing dual expression. Magnification, ×100 (
A,
C, and
F); ×200 (
B,
D, and
K–
P); ×400 (
E and
G–
I). Scale bars in
Q–
S: 20 μm.
