Inflammation-induced lymphangiogenesis
in the cornea arises from
CD11b-positive macrophages
J. Clin. Invest. Kazuichi Maruyama, et al. 115:2363 doi:10.1172/JCI23874 [
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Figure 6Characterization of PECs. (
A) Flow cytometric analysis of thioglycollate-activated adherent PECs. Percentage of PECs positive for CD11b is shown. The
x axis shows FITC fluorescence intensity. (
B) RT-PCR of CD11b
+ macrophages. Shown are CD11b macrophages stimulated with thioglycollate
VEGFR-3 (lane 1),
VEGFR-2 (lane 3), and
VEGF-C (lane 5) and their unstimulated counterparts (lanes 2, 4, and 6, respectively). The far left lane provides size markers. (
C) Multicolor FACS analysis of CD11b
+ macrophages. Percentages of double-positive cells are shown. (
D–
L) Immunocytochemistry of thioglycollate-activated adherent PECs. PE-conjugated CD11b (
D) and FITC-visualized Prox-1 (
E) staining. (
F) Overlay of
D and
E showing dual expression. PE-conjugated CD11b (
G) and FITC-visualized podoplanin (
H) staining. (
I) Overlay of
G and
H showing dual expression. PE-conjugated CD11b (
J) and FITC-visualized LYVE-1 (
K) staining; (
L) overlay of
J and
K showing dual expression. Magnification, ×400.
