Inflammation-induced lymphangiogenesis
in the cornea arises from
CD11b-positive macrophages
J. Clin. Invest. Kazuichi Maruyama, et al. 115:2363 doi:10.1172/JCI23874 [
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Figure 5Involvement of EPCs in lymphatic vascular development following corneal suture. (
A–
C) Tie2-LacZ mouse cornea. Cy2-visualized β-gal (
A) and Cy3-visualized LYVE-1 (
B) staining. (
C) Overlay of
A and
B showing dual expression. (
D–
F) Higher magnification view of area indicated by arrows in
A and
C. Cy2-visualized β-gal (
D) and Cy3-visualized LYVE-1 (
E) staining. (
F) Overlay of
E and
F showing dual expression. (
G–
I) LacZ expression at the blood vessel, not lymphatic in Tie2-LacZ chimera mouse cornea. Cy2-visualized β-gal (
G) and Cy3-visualized LYVE-1 (
H) staining. (
I) Overlay of
G and
H showing dual expression. (
J–
L) Higher magnification view of area indicated by arrow in
I. Cy2-visualized β-gal (
J) and Cy3-visualized LYVE-1 (
K) staining. (
L) Overlay of
J and
K showing dual expression. Scale bars: 80 μm (
A–
C and
G–
I), 40 μm (
D–
F), and 20 μm (
J–
L).
