Inflammation-induced lymphangiogenesis
in the cornea arises from
CD11b-positive macrophages
J. Clin. Invest. Kazuichi Maruyama, et al. 115:2363 doi:10.1172/JCI23874 [
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Figure 3Lymphatic vascular endothelium after PKP and suture placement. (
A–
F) Fluorescence microscopy. FITC-conjugated CD11b (
A) and Cy3-visualized LYVE-1 (
B) staining. (
C) Overlay of
A and
B showing dual expression of markers. FITC-conjugated CD11c (
D) and Cy3-visualized LYVE-1 (
E) staining. (
F) Overlay of
D and
E showing dual expression. (
G–
T) Confocal microscopy. FITC-conjugated CD11b (
G) and Cy3-visualized Prox-1 (
H) staining. (
I) Overlay of
G and
H showing dual expression. FITC-conjugated CD11b (
J) and Cy3-visualized Prox-1 (
K) staining. (
L) Overlay of
G and
K showing dual expression. (
M) Horizontal section image (bottom) and coronary section image (right) of the lymphatic vessel. (
N–
Q) EGFP bone marrow cells (green) appear to be part of the lymphatic endothelium or wall. EGFP expression (
N) and Cy3-visualized LYVE-1 (
O) staining. (
P) Overlay of
N and
O showing dual expression. (
Q) Horizontal section image (bottom) and coronary section image (right) of the lymphatic wall. (
R–
T) Three-dimensional image of a lymphatic vessel. EGFP expression (
R) and Cy3-visualized LYVE-1 (
S) staining. (
T) Overlay of
P and
R showing dual expression. Arrows indicate lymphatic vessel lumen. Magnification, ×100 (
A–
F). Scale bars: 16 μm (
G–
M); 8 μm (
N–
Q).
