Inflammation-induced lymphangiogenesis
in the cornea arises from
CD11b-positive macrophages
J. Clin. Invest. Kazuichi Maruyama, et al. 115:2363 doi:10.1172/JCI23874 [
Go to this article.]
Figure 1Initial (3–7 days) and late (56 days) phases of lymphangiogenesis after PKP. (
A) Hemangiogenesis (green) and lymphangiogenesis (orange) in the corneal stromal layer 3 days after PKP. The blood vessel structures appear to originate from cellular aggregates. (
B and
C) The dendritic-shaped cells aggregate and appear to anastomose with limbal lymphatic vessels 3 days after PKP. (
D and
E) A discrete lymphatic vessel (
D) and its 3D image (
E). (
F) Lymphatic vessels reach the interface (arrow) between donor and host 7 days after PKP. (
G) Diagram of eye structure after corneal transplantation showing location of blood vessels (green) and lymphatic vessels (orange). (
H) LYVE-1 (orange) and FITC-conjugated CD31 (PECAM-1; green) staining of explanted culture cornea after stimulation with IL-1β. (
I–
K) Fifty-six days after PKP, the inflammation is suppressed, and lymphatic vessels regress. Arrows indicate interrupted structure and appearance of dendritic-shape cells. Magnification, ×100 (
A,
F, and
I); ×200 (
B and
J); ×400 (
C,
H, and
K). Scale bar: 40 μm (
D).
