Cholesterol binding, efflux, and a PDZ-interacting domain of scavenger receptor–BI mediate HDL-initiated signaling
J. Clin. Invest. Chatchawin Assanasen, et al. 115:969 doi:10.1172/JCI23858 [Go to this article.]

Figure 8
SR-BI binds either plasma membrane or lysosomal membrane cholesterol. HEK 293 cells were transfected with SR-BI, CD/SRCT, LIMP-II/SRCT, SR-BI/LIICT, CD36/LIICT, or LIMP-II and labeled with [³H]photocholesterol; plasma membranes or lysosomal membranes were isolated; receptors were immunoprecipitated with antibody to the SR-BI C-terminal tail or to LIMP-II; and the [³H]photocholesterol associated with the receptor was assessed by autoradiography (top). The abundance of immunoprecipitated receptor was assessed by Western blotting (bottom), and the relative amount of [³H]photocholesterol-bound receptor versus total receptor was calculated.