Cholesterol binding, efflux, and a PDZ-interacting domain of scavenger receptor–BI mediate HDL-initiated signaling
J. Clin. Invest. Chatchawin Assanasen, et al. 115:969 doi:10.1172/JCI23858 [Go to this article.]

Figure 5
SR-BI C-terminal cytoplasmic domain is required for eNOS phosphorylation and activation. COS-M6 cells were transfected with eNOS cDNA and either wild-type SR-BI, SR-BII, or SR-BIΔ509 cDNA. For detection of eNOS phosphorylation (top), 24 hours after transfection the cells were starved in serum-free DMEM for another 24 hours, the cells were incubated with HDL (50 μg/ml) for 0–10 minutes, and cell lysates were analyzed by Western blotting using anti–phospho-eNOS–specific (Ser1179-specific) antibody (peNOS), anti-eNOS monoclonal antibody (eNOS), and anti–SR-BI (extracellular domain) polyclonal antibody (Receptor). For detection of eNOS activation (bottom), parallel sets of transfected cells were used, and [³H]L-arginine conversion to [³H]L-citrulline was measured during 15-minute incubations with control buffer or HDL (50 μg/ml). Values (mean ± SEM) are expressed as percent of HDL-stimulated activity above basal activity; n = 4. *P < 0.05 versus wild-type SR-BI.