Cholesterol binding, efflux, and a PDZ-interacting domain of scavenger receptor–BI mediate HDL-initiated signaling
J. Clin. Invest. Chatchawin Assanasen, et al. 115:969 doi:10.1172/JCI23858 [
Go to this article.]
Figure 4SR-BI is required for HDL-mediated eNOS activation. (
A) COS-M6 cells were transfected with eNOS cDNA and either SR-BI or CD36 cDNA. Forty-eight hours after transfection, eNOS activation was assessed by measuring [
3H]
L-arginine to [
3H]
L-citrulline conversion during 15-minute incubations with control buffer or HDL (50 μg/ml). *P < 0.05 versus basal;
†P < 0.05 versus SR-BI. (
B) COS-M6 cells were transfected with eNOS cDNA and either sham plasmid or SR-BI cDNA. Forty-eight hours after transfection, eNOS activation was assessed during 15-minute incubations with control buffer, HDL (50 μg/ml), or CD (2%). *P < 0.05 versus basal;
#P < 0.05 versus sham. Values (mean ± SEM) are expressed relative to basal activity designated as 100%; n = 4.
