Cholesterol binding, efflux, and a PDZ-interacting domain of scavenger receptor–BI mediate HDL-initiated signaling
J. Clin. Invest. Chatchawin Assanasen, et al. 115:969 doi:10.1172/JCI23858 [Go to this article.]

Figure 3
Interventions that decrease SR-BI efflux capacity attenuate eNOS stimulation. (A) BAECs were preincubated with control IgG or SR-BI blocking antibody for 30 minutes, and eNOS activation was assessed during 15-minute incubations with control buffer or HDL (50 μg/ml). (B) COS-M6 cells were transfected with eNOS cDNA and either wild-type SR-BI or AVI mutant SR-BI cDNA. Forty-eight hours following transfection, eNOS activation was assessed during 15-minute incubations with control buffer, HDL (20 and 50 μg/ml), or SUVs (250 or 500 μg/ml). Values (mean ± SEM) are expressed relative to basal activity designated as 100%; n = 4. *P < 0.05 vs. basal; ^†P < 0.05 versus no SR-BI Ab; ^#P < 0.05 versus wild-type SR-BI.