Cholesterol binding, efflux, and a PDZ-interacting domain of scavenger receptor–BI mediate HDL-initiated signaling
J. Clin. Invest. Chatchawin Assanasen, et al. 115:969 doi:10.1172/JCI23858 [Go to this article.]

Figure 2
Cholesterol-free Lp2A-I particles activate eNOS. (A) eNOS activation in BAECs was assessed by measuring [³H]L-arginine to [³H]L-citrulline conversion during 15-minute incubations with control buffer, HDL (20 μg/ml), or Lp2A-I particles with a molar POPC/apoA-I ratio of 80:1 (20 μg/ml). (B) eNOS activation in BAECs was assessed during 15-minute incubations with control buffer, HDL (1, 5, and 20 μg/ml), Lp2A-I particles with a molar POPC/apoA-I ratio of 40:1 (1, 5, and 20 μg/ml), or Lp2A-I particles with a molar POPC/apoA-I ratio of 80:1 (1, 5, and 20 μg/ml). White bars, 1 μg/ml; gray bars, 5 μg/ml; black bars, 20 μg/ml. (C) eNOS activation in BAECs was assessed during 15-minute incubations with control buffer, HDL (20 μg/ml), Lp2A-I particles with a molar POPC/cholesterol/apoA-I ratio of 80:0:1 (20 μg/ml), or Lp2A-I particles with a molar POPC/cholesterol/apoA-I ratio of 80:5:1 (20 μg/ml). Values (mean ± SEM) are expressed relative to basal activity designated as 100%; n = 4. *P < 0.05 versus basal; ^†P < 0.05 versus no cholesterol.