Cholesterol binding, efflux, and a PDZ-interacting domain of scavenger receptor–BI mediate HDL-initiated signaling
J. Clin. Invest. Chatchawin Assanasen, et al. 115:969 doi:10.1172/JCI23858 [Go to this article.]

Figure 1
Cholesterol efflux is required for HDL stimulation of eNOS. (A) eNOS activation was assessed in BAECs by measuring [³H]L-arginine to [³H]L-citrulline conversion during 15-minute incubations with control buffer (Basal), HDL (50 μg/ml), CD (2%), or cholesterol-loaded CD (Chol-CD, 2%). (B) eNOS activation in BAECs was assessed during 15-minute incubations with control buffer, HDL (5 and 20 μg/ml), or PC-HDL (5 and 20 μg/ml). Values (mean ± SEM) are expressed relative to basal activity, designated as 100%; n = 4. *P < 0.05 versus basal; ^†P < 0.05 versus no cholesterol. ^#P < 0.05 versus HDL.