Ferroportin1 is required for normal iron cycling in zebrafish
J. Clin. Invest. Paula G. Fraenkel, et al. 115:1532 doi:10.1172/JCI23780 [
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Figure 6Quantification of transcript levels by multiplex real-time PCR. RNA from liver (
A,
B, and
D) or intestine (
C) of 10- to 12-month-old adult zebrafish or pools of embryos (
E and
F) was used to generate cDNA templates for multiplex reactions amplifying the gene of interest and β-actin. Transcript abundance, normalized to β-actin expression, is expressed as a fold increase over a calibrator sample. (
A–
D) Expression of transferrin (
A), fpn1 (
B and
C), or hepcidin (
D) in adult WT, heterozygote, or mutant zebrafish treated with iron 6–8 months previously (+Fe) or never treated (no Fe). The calibrator sample was an uninjected WT adult; n = 6–9 individuals per cohort. *P = 0.01 compared with iron-injected WT zebrafish. **P < 0.0001 compared with iron-injected WT zebrafish. (
E and
F) At 48 hours after fertilization, embryos were anesthetized and injected with iron dextran (+) or anesthetized without iron injection (–). (
E) Eighteen-hour time course of hepcidin transcript abundance following iron dextran injection. The calibrator sample consisted of a pool of embryos 2 hours after iron injection; n = 2 pools of embryos per cohort.
‡P < 0.05 compared with uninjected age-matched embryos. (
F) Induction of hepcidin expression 18 hours after iron dextran injection of weh
Tp85c–/– or WT sibling embryos. The calibrator sample was an uninjected pool of embryos; n = 3 pools of embryos per cohort.
‡‡P < 0.01 compared with uninjected age-matched embryos.
