Ferroportin1 is required for normal iron cycling in zebrafish
J. Clin. Invest. Paula G. Fraenkel, et al. 115:1532 doi:10.1172/JCI23780 [Go to this article.]

Figure 5
Liver iron stores. Perls’ staining demonstrating accumulation of nonheme iron in the liver of uninjected WT (left), iron-injected WT (center), iron-injected weh^Tp85c–/– zebrafish (right) at 6 months of age (A) and 12 months of age (B). Magnification, ×40. Scale bar: 100 microns. (C) Higher magnification of area marked by rectangle in B. Magnification, ×160. Scale bar: 10 microns. (D) Nonheme iron levels were quantified (μg iron/g liver) using the bathophenanthroline sulfonate chromagenic assay. Due to the small size of the organs, livers were dissected from individual zebrafish at 12 months of age and pooled according to cohort; n = 2–4 pooled samples per cohort. *P = 0.02 compared with uninjected WT zebrafish.