Ferroportin1 is required for normal iron cycling in zebrafish
J. Clin. Invest. Paula G. Fraenkel, et al. 115:1532 doi:10.1172/JCI23780 [Go to this article.]

Figure 4
Kidney marrow iron stores. Iron-laden macrophages demonstrated by Perls’ staining in uninjected WT (left), WT treated with iron (center), and weh^Tp85c–/– zebrafish treated with iron (right) that were sacrificed at ages 6 months (A) and 12 months (B). Magnification, ×40. Scale bar: 100 microns. Iron-laden macrophages were located in the hematopoietic tissue that surrounds the pale staining renal tubules. (C) Higher magnification of area marked by rectangle in B. Magnification, ×160. Scale bar: 10 microns. (D) Average number of iron-laden macrophages per high-powered field (hpf) in each of the cohorts at 6 months (gray bars; n = 2–3) and 12 months (white bars; n = 4) of age. Zero indicates that no iron-laden macrophages were identified. *P = 0.029 compared with uninjected heterozygotes at 12 months; **P = 0.013 compared with uninjected heterozygotes at 12 months; ***P = 0.001 compared with uninjected heterozygotes at 12 months and P = 0.004 compared with uninjected WT zebrafish at 12 months.