Ferroportin1 is required for normal iron cycling in zebrafish
J. Clin. Invest. Paula G. Fraenkel, et al. 115:1532 doi:10.1172/JCI23780 [Go to this article.]

Figure 2
Marrow differentials obtained from the kidneys of adult zebrafish at 1 year of age. From each animal, 1 × 10⁵ kidney marrow cells were analyzed by FSC and SSC, according to a previously defined method (48). Representative flow cytometry plots are shown from an iron-injected WT (A) and a weh^Tp85c–/– (B) zebrafish. Four major populations were delineated: erythroid (red ellipse), lymphoid/erythroblast (yellow ellipse), myeloid (lilac ellipse), and the most immature precursor cells (blue ellipse). Shown next to each ellipse is the percentage of kidney marrow cells in each gate. (C) Mean percentages of kidney marrow cells in each of the major cell populations; n = 3–5 per cohort. *P = 0.006; **P < 0.0001 compared with iron-injected WT zebrafish. (D) Kidney marrow cytospins stained with Wright-Giemsa for iron-injected WT (left) compared with iron-injected weh^Tp85c–/– zebrafish (right). Open arrows indicate erythrocytes, while black arrow indicates 1 of 5 erythroblasts shown in the weh^Tp85c–/– zebrafish photomicrograph. Magnification, ×100. Scale bar: 10 microns.