Ferroportin1 is required for normal iron cycling in zebrafish
J. Clin. Invest. Paula G. Fraenkel, et al. 115:1532 doi:10.1172/JCI23780 [Go to this article.]

Figure 1
The phenotype of the weh mutation in adult zebrafish. (A) Photographs of an iron-injected WT zebrafish (left) compared with a pale, iron-injected homozygote (weh^Tp85c–/–) (right) at 1 year of age. (B) Erythrocytes from peripheral blood of iron-injected weh^Tp85c–/– (Mut + Fe; right) at 1 year of age exhibit pale cytoplasm and decondensed nuclei compared with WT zebrafish (WT no Fe; left) stained with Wright-Giemsa. Magnification ×100. Scale bars: 10 microns. (C and D) Erythroid indices were obtained with an ADVIA 120 automated analyzer at 6 months (gray bars, n = 3–5) and 12 months (white bars, n = 4–6) for each cohort: uninjected weh^Tp85c+/+ zebrafish (WT no Fe), iron-injected weh^Tp85c+/+ zebrafish (WT + Fe), uninjected weh^Tp85c+/– zebrafish (Het no Fe), iron-injected weh^Tp85c+/– zebrafish (Het + Fe), and iron-injected weh^Tp85c–/– zebrafish (Mut + Fe). MCV (C) is a measure of erythrocyte size, while CH (D) quantitates the amount of hemoglobin per erythrocyte. Data shown are means ± SE. ND, not done. *P < 0.0001 compared with 12-month-old iron-injected WT zebrafish.