Inhibition of TNF receptor 1 internalization by adenovirus 14.7K as a novel immune escape mechanism
J. Clin. Invest. Wulf Schneider-Brachert, et al. 116:2901 doi:10.1172/JCI23771 [Go to this article.]

Figure 5
14.7K expression does not affect NF-κB activation. (A) NIH 3T3 cells expressing PM-14.7K (left) or 14.7K (right) remained untreated or were treated with 10 ng/ml TNF for 20 minutes, and nuclear translocation of NF-κB was determined by immunofluorescence with an anti–NF-κB antibody (Rhodamine; red). Nuclei were counterstained with Hoechst dye 33258 (blue). (B) Cytoplasmic fractions of untreated PM-14.7K and 14.7K NIH 3T3 cells (lanes 1 and 2) or PM-14.7K and 14.7K NIH 3T3 cells (lanes 3 and 4) treated with 10 ng/ml TNF for 20 minutes were prepared and probed for degradation of IκBα. Anti-actin antibody served as a loading control.