Inhibition of TNF receptor 1 internalization by adenovirus 14.7K as a novel immune escape mechanism
J. Clin. Invest. Wulf Schneider-Brachert, et al. 116:2901 doi:10.1172/JCI23771 [
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Figure 3TNFR1 internalization is blocked by 14.7K. (
A) Internalization of TNFR1 was analyzed in NIH 3T3 cells expressing 14.7K or PM-14.7K by determining the amount of intracellular [
125I]TNF at the indicated time points after increasing the temperature to 37°C (mean ± SD;
n = 3). (
B–
G) Confocal laser scanning fluorescence microscopy of TNF receptor distribution on PM-14.7K or 14.7K cells labeled with biotin-TNF/streptavidin-FITC. Labeled TNF receptors were detected after 1 hour at 4°C on the cell surface (
B and
C) and intracellularly after 30 and 60 minutes incubation at 37°C in PM-14.7K cells (
D and
F). (
E and
G) In contrast, labeled TNF receptors remained on the cell surface of 14.7K cells even after 30 and 60 minutes incubation at 37°C, indicating inhibition of TNF receptor endocytosis by 14.7K.
