Inhibition of TNF receptor 1 internalization by adenovirus 14.7K as a novel immune escape mechanism
J. Clin. Invest. Wulf Schneider-Brachert, et al. 116:2901 doi:10.1172/JCI23771 [Go to this article.]

Figure 2
14.7K inhibits DISC assembly in NIH 3T3 and C127 cells. Magnetic fractions harboring labeled TNF-TNFR1 complexes were purified from PM-14.7K and 14.7K cells and immunoblotted with the antibodies indicated. Cellular protein extracts were used as expression controls (lysate). Magnetic (Magn.) TNFR1 fractions purified from NIH 3T3 (NIH) PM-14.7K cells demonstrate TNF-triggered recruitment of TRADD, FADD, and caspase-8 (A), whereas TNF treatment failed to induce the TNFR1-associated DISC in the corresponding magnetic fractions isolated from NIH 3T3 14.7K cells (B). TNF-dependent recruitment of TRADD, FADD, and caspase-8 to TNFR1 was also demonstrated in PM-14.7K C127 cells (C), while 14.7K C127 cells revealed a complete lack of DISC components after TNF treatment (D).