Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates
J. Clin. Invest. Hisashi Bashuda, et al. 115:1896 doi:10.1172/JCI23743 [Go to this article.]

Figure 1
Effect of anti-human CD80/CD86 mAbs on MLR in rhesus monkeys and functional activities of the cultured cells. (A) Freshly isolated CD4⁺ T cells (fresh cells) from peripheral blood of rhesus monkeys were cocultured with irradiated allogeneic PBMCs (stimulator) in the presence or absence of anti-human CD80/CD86 mAbs (10 μg/ml each) for 5 days. (B) Peripheral blood CD4⁺ T cells (fresh cells, white bars) or the cultured cells (black bars) were stimulated with donor or third-party splenocytes (gray bars). After 3 days culture or 5 days (for fresh cells), the responder cells were evaluated for their proliferation. (C and D) Dose-dependent suppression of the alloresponses of peripheral blood CD4⁺ T cells to donor-type stimulator cells by the cultured anergic cells. Cultures were set up with recipient CD4⁺ T cells (10⁵ cells/well) and donor (C) or third-party (D) stimulators (10⁵ cells/well) for 7 days. Cultured donor splenocytes (10⁵) or different numbers of the cultured cells were also added in some wells. In all assays, cells were incubated for 6 days and then pulsed with 10 μCi of [³H]thymidine for the last 18 hours and counted. The bars represent the mean of triplicate values and the brackets indicate the SD. rIL-2, recombinant IL-2.