Differential impact of prostaglandin H synthase 1 knockdown on platelets and parturition
J. Clin. Invest. Ying Yu, et al. 115:986 doi:10.1172/JCI23683 [Go to this article.]

Figure 1
Generation of PGHS1^Neo mice. (A) Targeting strategy. Numbers indicate known coding exons. Dashed lines indicate regions for homologous recombination; dotted lines represent fragments generated by PCR genotyping. TK, thymidine kinase. Restriction sites: A, ApaLI; B, BamHI; H, HindIII; X, XbaI. (B) Identification of PGHS1^Neo allele by Southern blot analysis. The 4.1- and 6.0-kb bands represent WT and targeted alleles, respectively, when ApaLI was used for DNA digestion, while the 5.3- and 3.7-kb bands represent the corresponding alleles with HindIII digestion. (C) PCR genotyping of tail biopsies from WT (317 bp) and PGHS1^Neo (752 bp) mice.