The IL-6–gp130–STAT3 pathway in hepatocytes triggers liver protection in T cell–mediated liver injury
J. Clin. Invest. Christian Klein, et al. 115:860 doi:10.1172/JCI23640 [Go to this article.]

Figure 3
Changes in liver nuclear phosphorylation of STAT1 and STAT3 during Con A–induced hepatitis. (A) Phosphorylation of STAT1 in wild-type and alfpCre gp130^LoxP/LoxP mice. Western blot analysis of STAT1 and STAT3 activation, as detected by phosphorylation of STAT1 and STAT3, in liver nuclear protein extracts of wild-type and alfpCre gp130^LoxP/LoxP mice. Animals were pretreated 3 hours before Con A administration with NaCl or IL-6 (200 μg/kg) by i.p. injection as indicated. Representative results of 3 independent experiments are shown. (B) Phosphorylation of STAT3 in wild-type and alfpCre gp130^LoxP/LoxP mice. (C) Double immunofluorescence of liver sections for P-STAT3 and CD11b expression. CD11b detects activated macrophages, NK cells, and monocytes. Mice were killed 2 hours after Con A injection. DAPI counterstaining was used to detect nuclei. (D) Double immunofluorescence of liver sections for P-STAT3 and CD11b expression. CD11b detects activated macrophages, NK cells, and monocytes. Mice were killed 4 hours after Con A injection. Representative areas are shown. Slides of 3 animals per group were analyzed. Magnification, ×400.