Alzheimer disease β-amyloid activity mimics cholesterol oxidase
J. Clin. Invest. Luigi Puglielli, et al. 115:2556 doi:10.1172/JCI23610 [Go to this article.]

Figure 3
Aβ:Cu²⁺-induced cholesterol oxidation correlates with toxicity in neuronal cultures. Hippocampal primary neurons were incubated with Aβ₄₂:Cu²⁺ (200 nM:400 nM) with or without TETA (50 μM) for the indicated times. (A) Effect on 4-cholesten-3-one generation. (B) Effect on LDH release in the conditioned media. *P < 0.05. (C) Effect on the cleavage of full-length PARP (f.l. PARP; 116 kDa) into 85-kDa apoptosis-related (PARP-85) isoforms of PARP. Data are mean ± SD of 3 experiments.