Alzheimer disease β-amyloid activity mimics cholesterol oxidase
J. Clin. Invest. Luigi Puglielli, et al. 115:2556 doi:10.1172/JCI23610 [Go to this article.]

Figure 2
Catalytic oxidation of cell-free and cellular cholesterol by Aβ:Cu²⁺. (A) Production of 4-cholesten-3-one by Aβ₄₂:Cu²⁺ (200 nM:400 nM) upon incubation for 60 minutes with increasing concentrations of nonoxidized cholesterol. Experiments were performed in triplicate. (B) Lineweaver-Burk transformation of the data shown in A. (C) Free cholesterol and (D) 4-cholesten-3-one in primary neurons after incubation for 2 days with Aβ₄₂ (200 nM) and Aβ₄₂:Cu²⁺ (200 nM:400 nM) with or without TETA (50 μM). Data are mean ± SD of 4 experiments. *P < 0.05.