Tregs and allergic disease
J. Clin. Invest. Douglas S. Robinson, et al. 114:1389 doi:10.1172/JCI23595 [Go to this article.]

Figure 1
Peripheral blood CD4⁺CD25⁺ T cells from atopic volunteers show reduced suppressive ability in allergen-stimulated T cell cultures. CD4⁺CD25^– T cells were separated from peripheral blood by immunomagnetic separation, then cultured with allergen extracts either alone or mixed with CD4⁺CD25⁺ T cells. Proliferation was assessed by incorporation of tritiated thymidine (shown as cpm), and IL-5 was measured in supernatants at day 6 of cultures by Luminex bead array. Data shown are means and standard errors for cpm and IL-5 from 9 separate nonatopic donors, showing almost complete suppression of responses of CD4⁺CD25^– T cells when CD4⁺CD25⁺ T cells were added. When these data were expressed as percentage suppression (reduction in counts in the mixed culture compared with those in CD4⁺CD25^– T cells alone), suppression was significantly less when cells were obtained from atopic donors or volunteers with hay fever studied in or out of season (IS or OS). Suppression out of season was significantly more than that seen in season but still significantly less than in nonatopic controls. Figure adapted from Lancet (54), with permission from Elsevier.