Identification of autoantibody clusters that best predict lupus disease activity using glomerular proteome arrays
J. Clin. Invest. Quan Li Zhen, et al. 115:3428 doi:10.1172/JCI23587 [Go to this article.]

Figure 1
Target Ags and specificity profiles of glomerular proteome arrays. (A) HydroGel slides were coated with different glomerular/GBM and nuclear Ags in duplicate as shown. Deramatan sulphate, deramatan sulphate proteoglycan; glom. extract, glomerular extract; HS, heparan sulphate. (B–G) Six commercially available mAbs specific for vimentin (B), hemocyanin (C), collagen IV (D), fibrinogen IV (E), elastin (F), and myosin (G) were added to 6 separate glomerular proteome arrays and developed using Cy5-labeled goat anti-mouse IgG/IgM in order to gauge the specificity of the Ag/Ab interactions on the glomerular proteome arrays. The Ags in B–G were arrayed as shown in A.