Pemphigus foliaceus IgG causes dissociation of desmoglein 1–containing junctions without blocking desmoglein 1 transinteraction
J. Clin. Invest. Jens Waschke, et al. 115:3157 doi:10.1172/JCI23475 [Go to this article.]

Figure 4
Localization of Dsg1, Dsg3, and plakoglobin at cell-to-bead contacts. Dsg1-coated beads (A–D) and Fc-coated beads (E and F) were immunostained following settlement on HaCaT cells for 30 minutes. Immunostaining for Dsg1 (B) showed localization of Dsg1 at the cell surface underneath most Dsg1-coated beads, which are visualized in A by corresponding phase contrast microscopic image. Note halo-like accumulation of cellular Dsg1 and plakoglobin around the bead attachment sites (arrows in B and D). Similarly, Dsg3 (C) was detected at cell-bead contacts. In contrast, no immunoreactivity for Dsg1 was found underneath Fc-coated beads visualized in a corresponding phase contrast figure (E and F). Scale bar: 10 μm for all panels (n = 5).