Pemphigus foliaceus IgG causes dissociation of desmoglein 1–containing junctions without blocking desmoglein 1 transinteraction
J. Clin. Invest. Jens Waschke, et al. 115:3157 doi:10.1172/JCI23475 [Go to this article.]

Figure 3
PF-IgG–induced cell dissociation is accompanied by loss of Dsg1 staining. After incubation of HaCaT cells with PF-IgGs (35 μg/ml, 24 hours), monolayers were double-stained for Dsg1 (B) and F-actin using Alexa-phalloidin (C). Intercellular gaps are indicated by arrows. Dsg1 is located along cell borders (A) and becomes strongly reduced in response to PF-IgGs (arrowheads in B). Scale bar: 40 μm for all panels (n = 5).