Pemphigus foliaceus IgG causes dissociation of desmoglein 1–containing junctions without blocking desmoglein 1 transinteraction
J. Clin. Invest. Jens Waschke, et al. 115:3157 doi:10.1172/JCI23475 [
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Figure 1Characterization of PF-IgGs and HaCaT cells. Dot blot immunodetection demonstrating that the mouse monoclonal antibody directed against Dsg1 (i) as well as PF1-IgGs (iii) but not control IgGs (ii) detected the recombinant fusion protein consisting of the complete extracellular domain of Dsg1 and the Fc-portion of human IgGs (Dsg1-Fc). Both Dsg1 and Dsg3 were detected in HaCaT cell lysates by the monoclonal anti-Dsg1 (a-Dsg1) antibody (iv–vi) and the monoclonal a-Dsg3 antibody (vii), respectively. Contents of Dsg1 constantly increased from 3 days (iv) and 5 days (v) up to 7 days after plating (vi), the time when monolayers were used for experiments. The data shown is for 1 representative experiment out of 3.
