Integrin α₄β₁–VCAM-1–mediated adhesion between endothelial and mural cells is required for blood vessel maturation
J. Clin. Invest. Barbara Garmy-Susini, et al. 115:1542 doi:10.1172/JCI23445 [Go to this article.]

Figure 7
Integrin α₄β₁ is required for angiogenesis. (A) Blood vessel branch points ± SEM in bFGF-, VEGF-A–, TNF-α–, or IL-8–stimulated CAMs treated with saline, anti–integrin α₄β₁ (P1H4), or control isotype-matched antibodies (P1F6, anti–integrin α_vβ₅). (B) Mice with 400 μg/ml VEGF-A implanted in the cornea were treated systemically with PS/2 or control IgG (rat anti-CD18, M18/2). Blood vessels identified by i.v. injection with FITC-labeled B. simplicifolia lectin. Magnification, ×200. Graph, area of neovascularization quantified by densitometry. Arrowheads indicate source of growth factor. (C) Mice subcutaneously injected with GF-reduced Matrigel containing 400 μg/ml rVEGF-A or bFGF were treated systemically for 5 days with PS/2 or control antibodies (rat anti-CD18, M18/2). Graph, CD31 positive vessels (arrowheads) per microscopic field in anti–integrin α₄β₁ (white bars) or control-treated tissues (black bars). Magnification, ×200. (D) Angiogenesis was initiated with GF-reduced Matrigel containing 400 ng/ml bFGF and 200 μg PS/2 or isotype-matched control antibodies. (E) Angiogenesis initiated as in D but with 50 μM EILDV or glutamic acid–isoleucine-leucine-glutamic acid–valine (EILEV) peptides incorporated in the Matrigel. (D and E) After 5 days, mice were injected intravenously with FITC-labeled B. simplicifolia; Matrigel plugs were homogenized in RIPA buffer, and fluorescence intensity was determined. *P < 0.05.