Integrin α₄β₁–VCAM-1–mediated adhesion between endothelial and mural cells is required for blood vessel maturation
J. Clin. Invest. Barbara Garmy-Susini, et al. 115:1542 doi:10.1172/JCI23445 [Go to this article.]

Figure 4
VCAM-1 is expressed on pericytes and integrin α₄β₁ on the endothelia of the same tumor vessels. Cryosections of murine MMTV-PyV-mT spontaneous breast carcinoma (left) and human breast carcinoma (middle and right) were immunostained with anti–integrin α₄β₁ (arrows) and anti–VCAM-1 (arrowheads) antibodies. Magnification, ×200 (left and middle panels); ×600 (right panels). Scale bar: 50 μm. Integrin α₄β₁ expression is generally restricted to the inner EC layer, and VCAM-1 expression is restricted to the outer cell layer of both small and large vessels.