Integrin α₄β₁–VCAM-1–mediated adhesion between endothelial and mural cells is required for blood vessel maturation
J. Clin. Invest. Barbara Garmy-Susini, et al. 115:1542 doi:10.1172/JCI23445 [Go to this article.]

Figure 3
Mural cells in developing vessels express VCAM. (A) Cryosections of murine MMTV-PyV-mT spontaneous breast carcinoma, human ductal breast carcinoma in situ, normal human breast, and normal murine breast were immunostained with anti-CD31 (green, arrowheads) and anti–VCAM-1 (red, arrows) antibodies. T, tumor. (B) Cryosections of murine MMTV-PyV-mT spontaneous breast carcinoma and normal mouse liver were immunostained to detect VCAM-1 (red) and desmin (green). Expression of VCAM-1 on mural cells is indicated by yellow (arrowheads). (C) Cryosections of murine MMTV-PyV-mT spontaneous breast carcinoma and normal mouse liver were immunostained to detect VCAM-1 (red) and SMA (green). Expression of VCAM-1 on mural cells is indicated by yellow (arrowheads). Scale bars: 50 μm.