Integrin α₄β₁–VCAM-1–mediated adhesion between endothelial and mural cells is required for blood vessel maturation
J. Clin. Invest. Barbara Garmy-Susini, et al. 115:1542 doi:10.1172/JCI23445 [Go to this article.]

Figure 2
Integrin α₄β₁ is accessible to the circulation in developing vessels. (A) Cryosections of subcutaneous xenograft HT29 colon carcinoma, M21 melanoma, and Lewis lung carcinoma tumors in nude mice were immunostained to detect integrin α₄β₁ (green) and CD31 (red). Overlap of expression is indicated by yellow (arrowheads). Ca, carcinoma. (B) Frozen sections of VEGF-A Matrigel plugs from mice injected intravenously with 50 μg doses of either PS/2 (left panels) or control rat anti–murine CD18 (M18/2, right panels) immunostained to detect anti-vWF (green) and bound rat anti-mouse antibodies (red). Yellow color in merged images indicates bound anti–integrin α₄β₁ or anti–integrin β2 (β2) antibody on endothelia. Magnification, ×200.