Integrin α₄β₁–VCAM-1–mediated adhesion between endothelial and mural cells is required for blood vessel maturation
J. Clin. Invest. Barbara Garmy-Susini, et al. 115:1542 doi:10.1172/JCI23445 [Go to this article.]

Figure 1
ECs in developing vessels express integrin α₄β₁. (A) Cryosections of saline-, bFGF-, or VEGF-A–stimulated chick CAMs were immunostained to detect vWF (red) and integrin α₄β₁ (α₄β₁) (green). Yellow color indicates overlap of expression patterns. (B) Cryosections of normal mouse breast, growth factor–reduced Matrigel supplemented with 400 μg/ml recombinant human VEGF-A, and MMTV-PyV-mT spontaneous breast carcinoma were immunostained to detect vascular EC CD31 (red) and integrin α₄β₁ (green). Yellow color indicates overlap of expression patterns. (C) Cryosections of human ductal breast carcinoma in situ were immunostained to detect vWF (red) and integrin α₄β₁ (green). Yellow color indicates overlap of expression patterns. In A–C, Arrowheads indicate blood vessels. Nl, normal. Magnification, ×200. Scale bar: 100 μm.