FGF-2 controls the differentiation of resident cardiac precursors into functional cardiomyocytes
J. Clin. Invest. Nathalie Rosenblatt-Velin, et al. 115:1724 doi:10.1172/JCI23418 [Go to this article.]

Figure 2
Characterization of the NMC population before differentiation. (A) Quantification by FACS analysis of neonatal and adult NMCs from FGF-2^+/+ (white bars) and FGF-2^–/– mice (black bars) expressing CD31, Flk-1, CD45, CD34, TER119, Mac-1, Gr-1, CD3, Sca-1, and c-kit after expansion in vitro (n = 5–12). (B) RT-PCR analysis of cardiac-specific gene expression as well as Sca-1, c-kit, and islet-1 in NMCs after expansion and before differentiation (BD), in neonatal cardiomyocytes (Neo CM) and in the adult heart (Ad heart) from either FGF-2^+/+ or FGF-2^–/– mice. C-actin, cardiac actin. (C) RT-PCR analysis of Snail and cadherin expression in expanded NMCs (BD), as well as in the neonatal (Neo heart) and adult hearts (Ad heart) from FGF-2^+/+ or FGF-2^–/– mice.