IL-1 mediates TNF-induced osteoclastogenesis
J. Clin. Invest. Shi Wei, et al. 115:282 doi:10.1172/JCI23394 [Go to this article.]

Figure 8
p38 MAPK mediates TNF- and IL-1–induced RANKL and IL-1RI expression. hMSCs were pretreated with SB203580 at the indicated concentrations overnight before exposure to vehicle (–) or (A) TNF (10 ng/ml) or (B) IL-1 (10 ng/ml) for 15 minutes. Cells were lysed and fractionated by SDS-PAGE, and activation of signaling molecules determined by immunoblot. ATF-2, activating transcription factor–2. (C) hMSCs were pretreated with decreasing concentrations of SB203580 for 30 minutes before exposure to PBS, TNF (10 ng/ml), or IL-1 (10 ng/ml) for 24 hours. RNA was extracted and analyzed by RT-PCR.