IL-1 mediates TNF-induced osteoclastogenesis
J. Clin. Invest. Shi Wei, et al. 115:282 doi:10.1172/JCI23394 [Go to this article.]

Figure 4
IL-1 mediates TNF-induced osteoclastogenesis in vitro. WT or IL-1RI–deficient, VCAM-1–positive murine marrow stromal cells were cocultured with bone marrow macrophages derived from p55/p75 TNF receptor–deficient mice in the presence of only the indicated cytokines. IL-1Ra (100 ng/ml) was added to half the cultures containing WT stromal cells. Medium was changed every 3 days. (A) After 7 days, the cells were stained for TRAP activity and (B) the number of multinucleated TRAP-positive cells determined. Values are mean ± SD for 6 determinations per group (**P < 0.01 vs. WT). OC, osteoclasts.