IL-1 mediates TNF-induced osteoclastogenesis
J. Clin. Invest. Shi Wei, et al. 115:282 doi:10.1172/JCI23394 [Go to this article.]

Figure 12
TNF and IL-1 induce IL-1RI expression in committed preosteoclasts in a p38-dependent manner. (A) Freshly isolated bone marrow macrophages (BMMs) and the same cells exposed to M-CSF plus RANKL for 3 days (preosteoclasts [pre-OCs]) were washed with PBS and then treated with TNF-α, IL-1, or RANKL for 24 hours. Total RNA was extracted with time and analyzed by RT-PCR for IL-1RI. (B) Preosteoclasts were pretreated with decreasing concentrations of SB203580 for 30 minutes before exposure to PBS (Control) or TNF (10 ng/ml), IL-1 (10 ng/ml), or RANKL (100 ng/ml) for 24 hours. RNA was extracted and analyzed by RT-PCR. (C) Preosteoclasts were treated with TNF (10 ng/ml) with or without IL-1Ra (100ng/ml) for 24 hours. Numbers in parentheses represent concentrations in ng/ml. RNA was extracted and analyzed by RT-PCR.