IL-1 mediates TNF-induced osteoclastogenesis
J. Clin. Invest. Shi Wei, et al. 115:282 doi:10.1172/JCI23394 [
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Figure 11IL-1 induction of osteoclast precursor differentiation occurs within a broad temporal window. (
A) WT marrow macrophages were cultured in the presence of M-CSF (50 ng/ml) and RANKL (10 ng/ml). IL-1 (1.25 ng/ml) or vehicle was added only once to each well. IL-1 addition occurred on sequential days from day 0 through day 4. TRAP stain was performed on day 5. (
B) Osteoclast number in each culture condition described in
A (
#P < 0.0001 vs. no IL-1 addition). (
C) WT marrow macrophages were cultured in the presence of M-CSF (50 ng/ml) plus RANKL (100 ng/ml). Total RNA was extracted with time and analyzed by RT-PCR for abundance of
IL-1RI and
IL-1RII mRNA.
