IL-1 mediates TNF-induced osteoclastogenesis
J. Clin. Invest. Shi Wei, et al. 115:282 doi:10.1172/JCI23394 [Go to this article.]

Figure 1
TNF induces RANKL gene expression by murine and human marrow stromal cells. (A) VCAM-1–positive and –negative murine marrow cells were exposed to PBS (Control), 1,25-dihydroxyvitamin D (Vit. D; 10^–8 M), or TNF (10 ng/ml) for 24 hours. (B) hMSCs were cultured for 1 week and then exposed to 1 or 10 ng/ml TNF for 24 hours. In both circumstances, total RNA was extracted and analyzed by RT-PCR. GAPDH mRNA served as loading control. Results are expressed as AU relative to control (arbitrarily set as 1).