Synaptopodin regulates the actin-bundling activity of α-actinin in an isoform-specific manner
J. Clin. Invest. Katsuhiko Asanuma, et al. 115:1188 doi:10.1172/JCI23371 [
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Figure 2Synaptopodin isoform expression in kidney and brain. (
A) Comparison of Synpo-long and Synpo-short. The red box shows the first 670 AA that are shared between both isoforms; the orange box shows AA 671–903 corresponding to the Synpo-alt fragment of Synpo-long. Synpo-alt contains a LPPPP motif (yellow) for ENA/VASP binding and a PPRPF motif (gray) for homer binding. (
B) RT-PCR with isoform-specific primers reveals the expression of both Synpo-short and Synpo-long mRNA in brain and kidney glomeruli (Glom). (
C) Western blotting (WB) with the polyclonal NT antibody against an epitope present in both Synpo-long and Synpo-short and the Synpo-alt–specific antibody (alt). At the protein level, only Synpo-long is expressed in the kidney, whereas only Synpo-short is expressed in the brain. (
D) Double-labeling immunofluorescence microcopy of Synpo-alt with the pan-synaptopodin monoclonal antibody G1 confirmed the expression of Synpo-long in podocytes (magnification, ×650).
