Insulin resistance reduces arterial prostacyclin synthase and eNOS activities by increasing endothelial fatty acid oxidation
J. Clin. Invest. Xueliang Du, et al. 116:1071 doi:10.1172/JCI23354 [Go to this article.]

Figure 2
Effect of FFAs on ROS production by endothelial cells. (A) Effect of FFAs on ROS production in arterial cells cultured in 5 mM glucose. Cells were incubated in 5 mM glucose without added insulin, plus the indicated concentrations of oleic acid (bars 1–4), and with oleic acid plus 3 nM insulin with and without pretreatment with 100 nM wortmannin (bars 5 and 6). Each bar represents the mean plus SEM of 4 separate experiments, each with n = 8. *P < 0.01 compared with cells incubated in 5 mM glucose alone. (B) Effect of FFAs on ROS production in retinal capillary endothelial cells cultured in 5 mM glucose. Cells were incubated in 5 mM glucose, 30 mM glucose, or 5 mM glucose plus oleic acid. Each bar represents the mean plus SEM of 4 separate experiments, each with n = 8. *P < 0.01 compared with cells incubated in 5 mM glucose alone.