Infiltration of COX-2–expressing macrophages is a prerequisite for IL-1β–induced neovascularization and tumor growth
J. Clin. Invest. Shintaro Nakao, et al. 115:2979 doi:10.1172/JCI23298 [Go to this article.]

Figure 4
The effect of Cl₂MDP-LIPs on IL-1β–induced angiogenesis. (A) FACS analysis of infiltrating cells on day 6, in IL-1β–implanted corneas from BALB/c mice that received Cl₂MDP-LIPs or PBS-LIPs i.v. and/or s.c. The cells were stained with PE-CD11b mAb and FITC–Gr-1 or FITC-F4/80 mAb. (B) Corneal neovascularization at the indicated time points in BALB/c mice receiving Cl₂MDP-LIPs or PBS-LIPs i.v. and/or s.c. The percentages of infiltrating cells in IL-1β–implanted corneas of Cl₂MDP-LIP– or PBS-LIP–treated mice were 4.75% ± 0.48% (i.v., CD11b⁺F4/80⁺), 13.2% ± 4.03% (i.v., CD11b⁺Gr-1⁺), 1.63% ± 0.30% (i.v. + s.c., CD11b⁺F4/80⁺), and 6.61% ± 0.93% (i.v. + s.c., CD11b⁺Gr-1⁺). (C) Neovascularization was quantified by area in mm² on day 4 (white bars) and day 6 (black bars). Bars show means ± SD of independent experiments (n = 3 or 4; *P < 0.01 and **P < 0.05 versus PBS-LIPs). (D) Corneal neovascularization induced with VEGF at the indicated time points after receiving Cl₂MDP-LIPs or PBS-LIPs (i.v. + s.c.). (E) Quantitative analysis of neovascularization on day 6. VEGF-induced corneal neovascularization in mice (n = 6) receiving Cl₂MDP-LIPs was inhibited compared with mice (n = 6) receiving PBS-LIPs. *P < 0.01 using the Student’s t test.